Vitamin B12

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vitamin B-12
EINECS 200-680-0
Molecular Formula: C63H89CoN14O14P++++
Molecular Weight: 1356.37000
Appearance and properties: red crystalline powder
Melting point: >300ºC
Stability: Hygroscopic. Keep cold and dry.
Storage conditions: 2-8ºC
Applications: Vitamin B12 is a water-soluble vitamin that plays a key role in maintaining the normal function of the brain and nervous system and the formation of blood flow.

Items of Analysis Standard of Analysis Test Results
Appearance Dark red crystals or dark red crystalline powder Conform
Identification A UV:Absorption maxima at 278±1nm,361±1nm and 550±2nm. Conform
A361nm/A278nm: 1.70~1.90
A361nm/A550nm: 3.15~3.45
Identification B TLC:The principal spot in the chromatogram obtained with the test solution is similar in position, colour and size to the principal spot in the chromatogram obtained with the reference solution. Conform
Loss on drying ≤10% 3.1%
Assay 97-102% 99.3%
Related substances Total impurities ≤3%  1.3%
Acetone ≤5000 ppm 619 ppm
The total aerobic microbial count ≤1000 cfu/g 65 cfu/g
The total combined yeasts/mould count ≤100 cfu/g <10 cfu/g
Conclusion Conforms to Factory Standard

Vitamin B12

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